Monoclonal antibody facility


Overview
Through the Monoclonal Antibody Facility, the Perkins provides high quality antibodies to researchers and commercial clients.

Our services range from production of antisera to manufacture of gram amounts of pure monoclonal antibodies.Kathy Davern - Head, Monoclonal Antibody Facility

Our specialty is in the production of custom monoclonal antibodies made with our high efficiency proprietary technology. We are one of the only suppliers with a strong track record in producing MAb to novel antigens that are particularly challenging.

The Facility is managed by Kathleen Davern.  Kathy has many years of experience in all aspects of monoclonal antibody production and has built on these strengths to expand both national and international capabilities.

Researchers, clinicians and commercial groups nationally and internationally have expressed their satisfaction with the value and efficiency of our services.

  • High efficiency monoclonal antibody production using the “Southern Cross”
  • Methods
  • Immunize various strains with GEX-biotin antigen
  • GEX (protein)-Biotin (small hapten)
  • Measure serum antibodies
  • Perform fusion
  • Compare:

antibody responses
Efficiency of MAb production

 

STRAIN

No of Fusions

Antibody Titre GEX

Antibody Titre Biotin

MAb +ve per fusion GEX

MAb +ve  per fusion Biotin

C57BL/6

14

3200

3200

16

2

SC 073

5

120,000

50,000

65

25

SC 018

4

40,000

25,000

54

9

SC 104

4

7500

7500

15

13

SC 261

4

16,000

15,000

36

7

SC 424

7

2000

200

30

19

SC 038

3

600

900

11

4

SC 381

4

25,000

25,000

33

11

SC 027

3

75,000

40,000

55

22

We use “Southern Cross” strain SC 073 routinely for superior monoclonal antibody production.



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