- Cancer and cell biology
- Cancer epigenetics
- Cell Signalling
- Laboratory for cancer medicine
- Iron Metabolism
- Liver Disease and Carcinogenesis
- Systems Biology and Genomics
- Targeted drug delivery, imaging and therapy
- Vascular Biology and Stromal Targeting
- Translational Cancer Research
- Clinical science
- Bioimaging Research and Innovation for Translational Engineering
- Centre for Clinical Research in Emergency Medicine
- Translational renal research
- Vascular Engineering
- Advanced Clinical and Translational Cardiovascular Imaging
- Molecular medicine
- Brain Growth and Disease
- Epigenetics and genomics
- Mitochondrial Medicine & Biology
- Molecular Endocrinology and Pharmacology
- Neurogenetic Diseases
- Synthetic Biology & Drug Discovery
- Cancer and cell biology
- Research centres
- Research facilities
- Clinical trials
- Laboratory Heads
Mitochondrial medicine and biology
Current research projects
Mitochondrial RNA-binding proteins and their role in mitochondrial gene expression.
Mitochondria play a fundamental role in cell and energy metabolism and consequently mitochondrial dysfunction can lead to severe multi-system disorders with wide range of clinical presentations that commonly include neurodegeneration, muscle defects and exercise intolerance. To understand these conditions better and identify therapeutic targets it is necessary to understand how gene expression is regulated within mitochondria, as some of the most significant gaps in our knowledge of mitochondrial function and disease are in the regulation of mitochondrial gene expression. Links between transcription and translation in mammalian mitochondria are not well understood.
Fig 1. Mitochondrial mRNAs are transcribed as part of long primary transcripts that generally encompass the entire mtDNA, therefore the ratios of the 11 mammalian mitochondrial mRNAs and their proteins are controlled post-transcriptionally. Little is known about how these 11 mRNAs are regulated in mammalian mitochondria. This is particularly important since tissue-, cell- and disease-specific variations in expression of mitochondrial RNAs has been observed, but cannot be explained at present. The basic components and mechanisms of transcription have recently been discovered, however the control of mRNA processing, translation and stability remains unclear.
We are interested in identifying mammalian mitochondrial RNA-binding proteins and investigating their role in RNA metabolism in cells. Discovery of proteins and the RNAs they bind may shed light on the regulation of gene expression in mammalian mitochondria. In addition, we are developing new methods for the identification of mitochondrial RNAs bound by the mitochondrial RNA-binding proteins that may regulate their expression in health and in disease.
Identification of mutations that cause mitochondrial disease
Mitochondrial diseases are progressive and debilitating multi-system disorders that occur as a result of mutations in nuclear or mitochondrial genes with no known cures to date. The clinical heterogeneity in mitochondrial disorders is complemented by genetic heterogeneity, where mutations in mitochondrial or nuclear genes cause similar phenotypes thus complicating mutation identification. We use next generation technologies to identify mutations in DNA from patients that suffer from mitochondrial diseases to identify the mutations that cause these diseases. We use patient cells to investigate how mutations in mitochondrial genes cause the molecular changes that cause mitochondrial and cellular dysfunction that leads to the disease pathology.